Carolina
The earliest directories seem to list it as a private house (dates are publication dates, which could at that time be more than 1 year earlier), though possibly with business from their homes, but this seems to change in the 1860s.
1841-50 George Newton Swinson, Surgeon (Eye hospital was a couple of doors away)
1855 George R.Bull
1862 Mrs Mary Louisa Pearson, stay & corset maker
1867 Thomas Ash & Co, zinc workers
Does that give you the information you require?